Association between Helicobacter pylori infection and pancreatic cancer. M Raderer, F Wrba, G Kornek, T Maca, DY Koller, G Weinlaender, M Hejna, W Scheithauer. Oncology 1998 Jan-Feb;55(1):16-19. 65% of 92 patients with pancreatic adenocarcinoma, and 69% of 30 with gastric cancer, were seropositive for HP, versus 45% of other controls (35 with colorectal cancer and 27 healthy volunteers). OR= 2.1 (1.1-4.1), p=0.035.

Detection of Helicobacter DNA in bile from bile duct diseases. IH Roe, JT Kim, HS Lee, JH Lee. J Korean Med Sci 1999;14:182-186. HP DNA was detected in 7/15 bile duct cancers, 2/6 pancreatic head cancers, and 3/11 cases of intrahepatic duct stones.

Helicobacter pylori seropositivity as a risk factor for pancreatic cancer. RZ Stolzenberg-Solomon, MJ Blaser, PJ Limburg, G Perez-Perez, PR Taylor, J Virtamo, D Albanes. J Natl Cancer Inst 2001 Jun 20;93(12):937-941. 82% of cases versus 73% of controls were positive for HP, OR 2.01 (1.09-3.70) with CagA+ strains. And: H pylori infection may be associated with exocrine pancreatic cancer. (Medscape - Reuters Health 2001 Jun 28. http://id.medscape.com/39766.rhtml link died.)

Helicobacter species ribosomal DNA in the pancreas, stomach and duodenum of pancreatic cancer patients. HO Nilsson, U Stenram, I Ihse, T Wadstrom. World J Gastroenterol 2006 May 21;12(19):3038-3043. "Patients with exocrine pancreatic cancer (n = 40), neuroendocrine cancer (n = 14), multiple endocrine neoplasia type 1 (n = 8), and chronic pancreatitis (n = 5) were studied. Other benign pancreatic diseases (n = 10) and specimens of normal pancreas (n = 7) were included as controls.... Helicobacter DNA was detected in pancreas (tumor and/or surrounding tissue) of 75% of patients with exocrine cancer, 57% of patients with neuroendocrine cancer, 38% of patients with multiple endocrine neoplasia, and 60% of patients with chronic pancreatitis. All samples from other benign pancreatic diseases and normal pancreas were negative. Thirty-three percent of the patients were helicobacter-positive in gastroduodenal specimens. Surprisingly, H. bilis was identified in 60% of the positive gastroduodenal samples. All gallbladder and ductus choledochus specimens were negative for helicobacter." "DNA of different Helicobacter species in the pancreas compared with gastroduodenal tissue was identified in patients who were Helicobacter-positive both in the stomach and pancreas. Moreover, many pancreas-positive PC patients were negative in stomach samples and vice versa, not supporting migration of helicobacter microorganisms colonizing the stomach to the pancreas in the studied PC patients."

Helicobacter pylori is implicated in biliary tract disease

Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis. JG Fox, FE Dewhirst, Z Shen, Y Feng, NS Taylor, BJ Paster, RL Ericson, CN Lau, P Correa, JC Araya, I Roa. Gastroenterology 1998 Apr;114(4):755-763. 9 of 23 gallbladder tissues were positive for Helicobacter by PCR. By phylogenetic analysis, five represented strains of H. bilis, two of "Flexispira rappini" (ATCC 49317), and one of H. pullorum.

Identification of Helicobacter pylori and other Helicobacter species by PCR, hybridization, and partial DNA sequencing in human liver samples from patients with primary sclerosing cholangitis or primary biliary cirrhosis. HO Nilsson, J Taneera, M Castedal, E Glatz, R Olsson, T Wadstrom. J Clin Microbiol 2000 Mar;38(3):1072-1076. 9/12 patients with primary sclerosing cholangitis and 11/12 with primary biliary cirrhosis were positive by PCR with Helicobacter genus-specific primers, versus 1/13 with noncholestatic liver cirrhosis and 0/10 normal livers (P = <0.00001).

Association between Helicobacter bilis in bile and biliary tract malignancies: H. bilis in bile from Japanese and Thai patients with benign and malignant diseases in the biliary tract. N Matsukura, S Yokomuro, S Yamada, T Tajiri, T Sundo, T Hadama, S Kamiya, Z Naito, JG Fox. Jpn J Cancer Res 2002 Jul;93(7):842-847. "Thirteen out of 15 (87%) Japanese and 11 out of 14 (79%) Thai patients with bile duct or gallbladder cancer tested positive for the presence of H. bilis in their bile. Eight out of 16 (50%) Japanese and 10 out of 26 (38%) Thai patients with gallstone and / or cholecystitis tested positive for H. bilis. Only 4 out of 14 (29%) subjects without biliary disease tested positive for H. bilis among the Japanese. Bile duct and gallbladder cancer showed significantly higher positive rates for H. bilis than did the non-biliary diseases among the Japanese (P < 0.01) and the odds ratios for bile duct or gallbladder cancer with H. bilis in comparison with gallstone and / or cholecystitis were 6.50 (95%CI 1.09 - 38.63) in the Japanese and 5.86 (1.31 - 26.33) in the Thai patients."

Helicobacter pylori and the risk of benign and malignant biliary tract disease. M Bulajic, P Maisonneuve, W Schneider-Brachert, P Muller, U Reischl, B Stimec, N Lehn, AB Lowenfels, M Lohr. Cancer 2002 Nov 1;95(9):1946-1953. 89 patients: 63 with biliary calculi, 15 with carcinoma of the biliary tract, and 11 with neither gallstones nor carcinoma. "Patients with gallstones were 3.5 times as likely to have H. pylori in the bile compared with patients in a control group (95% confidence interval [95%CI], 0.8-15.8; P = 0.100), and H. pylori was 9.9 times more frequent in patients with biliary tract carcinoma compared with patients in the control group (95%CI, 1.4-70.5; P = 0.022)."

Association of the presence of Helicobacter in gallbladder tissue with cholelithiasis and cholecystitis. CP Silva, JC Pereira-Lima, AG Oliveira, JB Guerra, DL Marques, L Sarmanho, MM Cabral, DM Queiroz. J Clin Microbiol 2003 Dec;41(12):5615-5618. Nested PCR of 16S rRNA genes in gallbladder tissue and bile from 46 Brazilian subjects with and 18 without cholelithiasis. "When the logistic regression model was applied in the multivariate analysis, cholelithiasis remained independently associated with increasing age (P = 0.002; odds ratio [OR] = 1.07; 95% confidence interval [CI] = 1.03 to 1.12), female gender (P = 0.02; OR = 5.68; 95% CI = 1.38 to 23.49), and the presence of H. pylori DNA in the gallbladder tissue (P = 0.009; OR = 14.72; 95% CI = 1.97 to 108.90)."

cast 12-30-06